Hi,
The results of my data after running modkit extract full does not match my reference.
From my reference, position 138 and 140 is T and 139 is C.
However, from the results of modkit extract full some read that contains position139 is identified as T in the out.
I see the same issue across the whole transcript, some positions are wrongly identified as T.
Looking at some of the reads and comparing the query_kmer with the reference sequence, it seems to be caused by an indels that is unmapped to the reference.
This is part of my reference:
135-144 (starts from 1)
cgcctctggc
For my downstream analysis, can I just discard the positions that have the wrong reference?
Below is the output of my results:
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| 5f0af38a-7492-48aa-a3de-1ed2ee404383 |
181 |
138 |
gene |
+ |
+ |
+ |
2 |
62 |
9 |
3149 |
3243 |
0.9863281 |
17802 |
13 |
. |
TCGCCTCTGGC |
T |
T |
FALSE |
0 |
3149 |
| f41ca671-d4c5-42d6-8ee6-1b954a8d964f |
127 |
139 |
gene |
+ |
+ |
+ |
9 |
16 |
22 |
3149 |
3140 |
0.9082031 |
17802 |
7 |
. |
CGCCCTTGGCC |
T |
T |
FALSE |
0 |
3149 |
| ad238b5c-a836-478d-b239-a65408a0af2c |
128 |
139 |
gene |
+ |
+ |
+ |
1 |
39 |
9 |
3149 |
3185 |
0.9042969 |
17802 |
11 |
. |
CGCCTTCGGCC |
T |
T |
FALSE |
0 |
3149 |
| bffc67f7-c72c-4a60-a7b4-cb0b857fb67d |
129 |
139 |
gene |
+ |
+ |
+ |
3 |
42 |
12 |
3149 |
3171 |
0.9902344 |
17802 |
9 |
. |
CGCCTTCGGCC |
T |
T |
FALSE |
0 |
3149 |
| efa15c98-4958-40a0-9aef-d730efe32625 |
63 |
140 |
gene |
+ |
+ |
+ |
0 |
39 |
76 |
3149 |
3079 |
0.9941406 |
17802 |
31 |
. |
GCCTCTGGCCC |
T |
T |
FALSE |
0 |
3149 |
Hi,
The results of my data after running modkit extract full does not match my reference.
From my reference, position 138 and 140 is T and 139 is C.
However, from the results of modkit extract full some read that contains position139 is identified as T in the out.
I see the same issue across the whole transcript, some positions are wrongly identified as T.
Looking at some of the reads and comparing the query_kmer with the reference sequence, it seems to be caused by an indels that is unmapped to the reference.
This is part of my reference:
135-144 (starts from 1)
cgcctctggc
For my downstream analysis, can I just discard the positions that have the wrong reference?
Below is the output of my results:
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