I noticed two possible issues in the Wimley–White hydrophobicity data:
The normalized LEU value appears to be incorrect. Most values were calculated using:
normalized = -original / 2.41
Therefore, LEU should be:
-(-0.69) / 2.41 = 0.28630705
However, its current value is 0.33609959, which is the normalized value for ILE.
The description says that +1 is the highest hydrophobicity, but the current normalization produces a maximum of approximately 0.3361. Thus, the values fall within [-1, 1] but do not span the full interval.
Also, the source values appear to come specifically from the Wimley–White Octanol − Interface scale rather than the octanol scale alone.
Reference: https://blanco.biomol.uci.edu/hydrophobicity_scales.html
Could you please confirm whether the intended normalization is division by the largest absolute value or min–max scaling to exactly [-1, 1]?
I noticed two possible issues in the Wimley–White hydrophobicity data:
The normalized LEU value appears to be incorrect. Most values were calculated using:
normalized = -original / 2.41
Therefore, LEU should be:
-(-0.69) / 2.41 = 0.28630705
However, its current value is 0.33609959, which is the normalized value for ILE.
The description says that +1 is the highest hydrophobicity, but the current normalization produces a maximum of approximately 0.3361. Thus, the values fall within [-1, 1] but do not span the full interval.
Also, the source values appear to come specifically from the Wimley–White Octanol − Interface scale rather than the octanol scale alone.
Reference: https://blanco.biomol.uci.edu/hydrophobicity_scales.html
Could you please confirm whether the intended normalization is division by the largest absolute value or min–max scaling to exactly [-1, 1]?